Do you put your hotbar in a certain order?

Title.
I always put a few things in certain places, no matter how the build is, they go there. (put a blank space if you don't put anything specific there).
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I do put certain items in certain order. I also do this. All 3-5 slots are used for offensive/defensive items. Passive items like, WSS, RED ORB, etc are setup so I hit START, X,X,X. , and if I have WSS in the first spot, 4 quick taps and I lay the sign, it's a pain cycling through TOTALLY USELESS items , in a crisis situation. Hehe. Reorder your items and you can semi- increase your 5 Quick slots, to 5 quick & 2-3 semi quick picks.

1. Estus
2. Humanity
3. Divine Blessing
4. Elizabeth Mushroom
5. Black Firebomb

The only thing for me that's pretty much guaranteed to be on my bar is the Estus. All other stuff 100% depends on what I'm doing.

If I expect to be poisoned I'll put some moss in there.
If I expect to run out of Estus I'll put my humanities in there.
If I'm hosting PvP I'll put the dried finger in there.
If I'm going to invade I'll put in the invasion item and humanities.
If I'm playing on a pure melee build and think I might need a ranged option, I'll put some poisoned knives or fire bombs in there.
And if I'm playing as a dragon, the dragon stones.

1. estus (I always remove it when I'm a phantom)
2. green blossom
3. repair powder or gold/charcoal pine resin and at low SLs firebombs
4. situational, though usually empty
5. humanity

I very rarely use a hotbar spot for an invasion item, but I usually switch it for estus after I've cleared the enemies or if I'm not going to bother engaging. I also organize my main menu and weapons in a certain order so I can get to them quickly/blind as well. I wonder how much of my menu I could re-create from memory alone...

1:Humanity
2:Elizabeths Mushroom
3:Divine Blessing
4:Red Moss Clump
5:Dried Finger or Black Seperation Crystal

Estus
Humanity
WSS
Red Orb/Blue Orb/Purple Moss
Poison Knives/Lloyds Talismans

1.
2.
3. Green Blossom
4. Humanity
5. Homeward Bones

The guys with Divine Blessings are obviously hackers...

depends;
1. always eustus, 5. always white chalk. 2 3 and 4 depends on whether host or phantom. always empty unless i'm in blight town. hum in slot 2 as phantom. reason chalk is in slot 5 is if i'm solo, i can tell when threatened by invader so i get ready for them.

For PvE:
1. Estus
2. Homeward Bone
3. Blooming Moss

For PvP duels:
1. Grass
2. resin buff?
3. Blooming Moss
4. Blood Moss
I also have Humanity at the bottom of my menu, and my summon sign at the top of my menu, so I can access it fast.

For Invasions:
1. Grass
2. Lloyd's
3. Resin buff?
4. Moss (I change it quickly depending on the setup of the host/phantom
5. Dung Pie.

PvP, I have no set order... PvE however, order:
1. Estus, 2. Orange Sign, 3. White Sign, 4. Homeward Bone, 5. Usually Purple MC, but interchangable.