In the first Deus Ex on PlayStation I wandered into the Women's Washroom by mistake (I play a lot of RPG's, so I obsessively open every door), this woman comes in and starts haranguing me and calling me a pervert (I am female in real life but I felt really embarassed and actually mumbled sorry at the screen-go figure). That wasn't the real magical bit though. That happened when I, as JC went in to see the bossman and he said 'Oh, and by the way Denton, keep out of the Womens' Washroom etc...' I was thunderstruck and remember thinking'she told on me, the b***h, I should have shot her!
Another time that sticks in my mind was playing a Halo game. I'm almost sure it was ODST. Anyway I'm on a highway helping the guy played by Nathan Filian to get this tank to our destination and I leap off and shoot one of those little grunts and another one pops up and shouts 'he was my fwend'. Honestly, I felt really awful and hesitated...just long enough for the litttle sod to lob a plasma grenade at me and kill me.
And last but not least, playing Gears of War 2, I was driving a tank through a cave. It was really dark and I was going dead slow because in Gears you're never sure of what's around the corner. After about a minute one of the guys with me shouted 'Mummy, Daddy, are we there yet!' It really cracked me up. Anyone else had moments like this?